Journal: Discover Oncology
Article Title: Flavokawain A suppresses the malignant progression of neuroblastoma in vitro depending on inactivation of ERK/VEGF/MMPs signaling pathway
doi: 10.1007/s12672-024-01568-y
Figure Lengend Snippet: FKA treatment suppresses the proliferative and clone-forming abilities of NB cells. A The structure of FKA. B HUVECs were treated with 0, 12.5, 25, 50 μM FKA for 48 h. The viability of HUVECs was detected by CCK-8 assay. C Human NB cell line (SK-N-SH) was treated with 0, 12.5, 25, 50 μM FKA for 48 h. The viability of SK-N-SH cells was detected by CCK-8 assay. D SK-N-SH cells were treated with 0, 12.5, 25, 50 μM FKA for 48 h. The clone-forming ability of SK-N-SH cells was detected by colony formation assay. *** p < 0.001
Article Snippet: Human NB cell line (SK-N-SH) and human umbilical vein endothelial cells (HUVECs) purchased from iCell Bioscience (Shanghai, China) were cultured in DMEM (Gibco, CA, USA) supplemented with 10% FBS and 1% penicillin–streptomycin at 37 °C in a humidified incubator containing 5% CO 2 .
Techniques: CCK-8 Assay, Colony Assay